Fig. 4. Effect of linoleic acid (LA) on inflammatory responses of Swan71 cells. A) There was no significant difference in IL-6 mRNA expression between LA treated group and vehicle control group (n=6). B) 300 and 500 μM of LA significantly decreased IL-6 release in cell culture supernatant as measured by ELISA (n=3). C) 500 μM of LA of significantly increased mRNA expression of IL-8 (n=6). D) 300 μM of LA of significantly decreased IL-8 protein expression (n=3). E-F) All three doses of LA significantly increased prostaglandins E2 (PGE2) and leukotrienes B4 (LTB4) in cell culture supernatant (n=5). Data expressed as mean ±SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 compared to vehicle control (BSA+ EtOH).